Fishes, Free Full-Text
Por um escritor misterioso
Descrição
Non-specific cytotoxic cell l (NCC) is a kind of important lymphocyte participating in the non-specific immune response in teleost. Non-specific cytotoxic cell receptor protein 1 (NCCRP-1) is a receptor molecule on the surface of NCC and plays an important role in mediating the activity of NCC. However, there are few reports on which signal molecule could transmit signals through NCCRP-1. In this study, yeast two-hybrid library of tilapia liver and head kidney was constructed, and a transferrin from Oreochromis niloticus (On-TF) with interaction protein sequence was obtained by screening the library with bait vector NCCRP-1 of Oreochromis niloticus (On-NCCRP-1). Then, the open reading frame (ORF) of On-TF was cloned, which had 2088 bp in length, encoding polypeptides of 695 amino acids. The deduced amino acid sequence was highly homologous to teleost and similar to mammalian TF, containing two TR_FER domains (25-343th aa and 344-686th aa) for binding iron ions. Furthermore, a point-to-point yeast two-hybrid method was used to further verify whether On-NCCRP-1 could bind to On-TF. The recombinant On-TF (rOn-TF) protein was purified by prokaryotic expression system. In vitro experiments showed that rOn-TF could up-regulate the expression of killing effector molecule of NCC by On-NCCRP-1, and rOn-TF-activated NCCs showed a significantly improved ability to kill FHM cells, indicating that rOn-TF could regulate the NCC signaling pathway through NCC receptor molecule On-NCCRP-1. The results provide a more theoretical basis for understanding the regulation mechanism of NCC activity.
Aquaculture, Fish and Fisheries - Wiley Online Library
National Audubon Society field guide to fishes : Gilbert, Carter Rowell, 1930- : Free Download, Borrow, and Streaming : Internet Archive
When an occluding fish shoal is uniformly distributed through the water
Todays Catch Is A Stringer Full Of Multicolored Fish. Sure To Be A Hit With Any Angler. Royalty Free SVG, Cliparts, Vectors, and Stock Illustration. Image 40752084.
Cell - What a Walking Fish Can Teach Us About Human Evolution Read the original free article here
Free the Fish! New Clare V selling quickly🐟 #newarrivals #clarev
FISH IS FISH - ENGLISH : LEO LIONI : Free Download, Borrow, and Streaming : Internet Archive
Biology, Free Full-Text
PDF] Read Free Take One Fish: The New School of Scale-to-Tail Cooking and Eating Full Pages New! / X
Tropical Fish Hobbyist - August 2008 - page 127
Fish SVG Bundle Fishing SVG Fish & Trees SVG Fish Cut File - Israel
de
por adulto (o preço varia de acordo com o tamanho do grupo)
